Antibacterial activity of therapeutic agent-immobilized nanostructured TiCaPCON films against antibiotic-sensitive and antibiotic-resistant Escherichia coli strains
نویسندگان
چکیده
The development of flexible and low-cost methods surface functionalization to fight infection at the early stage is an urgent scientific task. Herein, polymerization in low-pressure plasma rich COOH species carbodiimide chemistry were utilized immobilize four different therapeutic agents (antibiotic (gentamicin), antimicrobial peptide (indolicidin), anti-adhesive molecules (heparin) nitroxide radicals (2,2,5,5-tetramethyl-3-carboxyl-pyrrolidine-1-oxyl)) on nanostructured biocompatible TiCaPCON films impart antibacterial characteristics. polymers deposited from COOH-rich showed decent stability phosphate-buffered saline solution successfully used for immobilization via ionic or covalent bond. bactericide attachment was proved by FTIR spectroscopy XPS analysis. All samples with grafted hydrophilic water contact angle values range 26–56°. Bactericide release tests indicated maximum concentration case immobilization. In immobilization, fast initial observed over 24 h followed slower leaching next 48 (heparin), 96 (gentamicin). pH-sensitive polymer degradation gentamicin demonstrated. bactericide-linked noticeable reduction antibiotic-sensitive E. coli U20 strain and, except indolicidin-immobilized samples, effectively inhibited growth antibiotic-resistant K261 their 104 CFU/mL. not only exhibited highest activity against cells (100% after 8 h), but also prevented biofilm formation.
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ژورنال
عنوان ژورنال: Surface & Coatings Technology
سال: 2021
ISSN: ['1879-3347', '0257-8972']
DOI: https://doi.org/10.1016/j.surfcoat.2020.126538